Journal: PLoS ONE

Article Title: Targeting Homologous Recombination in Notch-Driven C . elegans Stem Cell and Human Tumors

doi: 10.1371/journal.pone.0127862

Figure Lengend Snippet: RNAi of DDR orthologs detects genes that enhance ar202 tumor radiosensitivity.

Article Snippet: The IDs of C . elegans gene expression assay are: mre-11 —Ce02480998_g1; rad-51 —Ce02458920_g1; atl-1 —Ce02479867_g1; mus-101 —Ce02413322_g1; cku-80 —Ce02445546_g1; lig-4 —Ce02449042_g1; hsr-9 —Ce02412427_g1; rad-50 —Ce02482582_g1; mec-7— Ce02497588_g1.

Techniques: Ubiquitin Proteomics, Histone Deacetylase Assay

Journal: Gene Therapy

Article Title: First use of gene therapy to treat growth hormone resistant dwarfism in a mouse model

doi: 10.1038/s41434-022-00313-w

Figure Lengend Snippet: a RT-PCR gel of mGHR mRNA expression. 18S rRNA housekeeping gene was used as loading control. b Western blot of mGHR protein expression. Actin housekeeping gene was used as loading control. c Immunofluorescence staining of mGHR expression, scale bars are 50 µm and nuclei were stained with DAPI. HepG2 cells were transfected with pAAV-HLP-mGHR consisted of hybrid liver-specific promoter (HLP) driving the expression of mGHR. Untransfected control and cells transfected with pAAV-HLP-Luc expressing luciferase gene were used as negative control. Analyses were performed 48 h post-transfection.

Article Snippet: The mGHR PCR fragment was amplified from cloning vector MG50043-M (Sino Biological, Beijing, China) containing mouse growth hormone receptor/GHR/GHBP transcript variant 1 gene ORF cDNA clone using forward primer with introducing NotI site: 5’-ATAAGAATGCGGCCGCACCATGGATCTTTGTCAGGTCTTC and reverse primer with introducing XhoI site: 5’-CCGCGCTCGAGCTACTGCATGATTTTGTTCAGTTG.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Immunofluorescence, Staining, Transfection, Luciferase, Negative Control